Research Paper Volume 12, Issue 3 pp 2440—2452

HO-1 induced autophagy protects against IL-1 β-mediated apoptosis in human nucleus pulposus cells by inhibiting NF-κB

Figure 2. IL-1β treatment inhibits autophagy and enhances apoptosis in the human NPCs. (A) Western blot shows the proteins expressions of p-P65, Bax/Bcl-2, Cleaved caspase 3 and LC3-II/I in NPCs after IL-1β (10ng/mL) treatment with or without 1% FBS. Note: The data represent mean ± SD of three experiments; ****p<0.0001, ***p<0.001 and *p<0.05. (B) Flow cytometry shows the percentage of apoptotic cells in NPCs after IL-1β treatment with or without 1% FBS. Note: The data represents mean ± SD of three experiments; ***p<0.001.