Figure 3. TUSC8 affects breast cancer cell metastasis through regulating the expression of epithelial–mesenchymal transition (EMT) related markers. (A) Over-expression of TUSC8 down-regulated the expression of mesenchymal related markers (ZEB1, TWIST, SNAI1 and Vimentin) in breast cancer cell lines SK-BR-3 and MDA-MB-435 by western blot. (B) Knock-down of TUSC8 up-regulated the expression of mesenchymal related markers (ZEB1, TWIST, SNAI1 and Vimentin), while down-regulated the expression of epithelial related marker (E-cadherin) in breast cancer cell lines MCF-7 and HCC1937 by western blot. The asterisks (*, **) indicate a significant difference (p < 0.05, p < 0.01) respectively.