Research Paper Volume 12, Issue 3 pp 2974—2991

Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis

Figure 7. TUSC8 inhibits breast cancer cell invasive capacity partly through miR-190b-5p-MYLIP axis. (A) Over-expression of TUSC8 significantly increased the mRNA and protein levels of MYLIP in SK-BR-3 cells. (B) Knock-down of TUSC8 drastically suppressed the mRNA and protein levels of MYLIP in HCC1937 cells. (C) Over-expression of miR-190b-5p or inhibition of MYLIP partly abolished the reduced cell invasive capacities mediated by TUSC8 over-expression in SK-BR-3 cells. (D) Inhibition of miR-190b-5p or MYLIP over-expression partly rescued the enhanced cell invasive capacities mediated by TUSC8 knock-down in HCC1937 cells. (E) Over-expression of miR-190b-5p or inhibition of MYLIP partly abolished the EMT related markers expression mediated by TUSC8 over-expression in SK-BR-3 cells. (F) Inhibition of miR-190b-5p or MYLIP over-expression partly rescued the EMT related markers expression mediated by TUSC8 knock-down in HCC1937 cells. The asterisks (*, **, ***) indicate a significant difference (p < 0.05, p < 0.01, p < 0.001) respectively.