Figure 3. BBR induces XIAP-mediated apoptosis through PIM-2. (A) Representative western blot analysis of PIM-2 expression in ALL cells EU-4 and EU-6 treated with different concentration of BBR. (B) PIM-overexpressed EU-4 and EU-6 cells were treated with 50 μM BBR for 24 h. The transfection efficiency was determined using western blot. (C) Cell apoptosis was measured by TUNEL assay. (D) PIM-2 overexpressed EU-4 and EU-6 cells were treated with 50 μM BBR in the presence or absence of XIAP antagonist ASTX660 for 24 h followed by treatment with 50 μM BBR for 24 h. Cell apoptosis was detected by TUNEL assay. Results are expressed as mean ± SD.**p<0.01, compared to the vector or control group.