Research Paper Volume 12, Issue 5 pp 4111—4123

Long noncoding RNA LINC00461 induced osteoarthritis progression by inhibiting miR-30a-5p

Figure 7. Overexpression of LINC00461 enhanced chondrocyte proliferation, cell cycle progression, inflammation, and ECM degradation by downregulating miR-30a-5p. (A) Cell proliferation was measured by CCK-8 assay. (B) Elevated expression of miR-30a-5p inhibited IL-8 in LINC00461-overexpressing chondrocytes. (C) The expression of IL-10 was determined by qRT-PCR assay. (D) Ectopic expression of miR-30a-5p decreased MMP-2 expression in LINC00461-overexpressing chondrocytes. (E) The expression of MMP-3 was measured by using qRT-PCR assay. (F) The expression of MMP-13 was measured by using qRT-PCR assay. (G) miR-30a-5p overexpression enhanced collagen II expression in LINC00461-overexpressing chondrocytes. *p<0.05 and **p<0.01.