Research Paper Volume 12, Issue 4 pp 3713—3729

Identification of a lathyrane-type diterpenoid EM-E-11-4 as a novel paclitaxel resistance reversing agent with multiple mechanisms of action

Figure 4. Effects of EM-E-11-4 on the accumulation of Flutax-1 and Rhodamine 123 in A549 and A549/Tax cells. (A) Effects of EM-E-11-4 on the accumulation of Flutax-1 in A549 and A546/Tax (×100) cells. (A-a) 5 μM Flutax-1 in A549 cells; (A-b) 5 μM Flutax-1 in A549/Tax cells; (A-c) 5 μM Flutax-1 and 2.5 μM EM-E-11-4 in A549/Tax cells; (A-d) 5 μM Flutax-1 and 5 μM EM-E-11-4 in A549/Tax cells; (A-e) 5 μM Flutax-1 and 10 μM EM-E-11-4 in A549/Tax cells; (A-f) 5 μM Flutax-1 and 10 μM verapamil in A549/Tax cells; (A-g) quantitative data for the fluorescence intensity. ## p<0.01 vs. A549 cells control, ** p<0.01 vs. A546/Tax cells control. (B) Effects of EM-E-11-4 ond the accumulation of Rhodamine 123 in A549 and A549/Tax cells. (B-a) The levels of Rhodamine 123 assayed by flow cytometry in A549 cells; (B-b) The levels of Rhodamine assayed by flow cytometry in A549/Tax cells; (B-c) The relative values of Rhodamine 123 in A549 cells; (B-d) The relative values of Rhodamine 123 in A549/Tax cells. Columns represent the means±SD values for Rhodamine 123 obtained from three individual experiments. * p<0.05 and ** p<0.01 vs. control (10μM EM-E-11-4).