Research Paper Volume 12, Issue 4 pp 3862—3879

Acetate attenuates perioperative neurocognitive disorders in aged mice

Figure 7. The protective effects of acetate depended on GPR43 expression (n=3 independent experiments). After being treated with si-GPR41 or si-GPR43 for 48 h, BV2 cells were stimulated for 6 h with LPS, with or without subsequent acetate treatment, and the supernatants were examined by ELISA. (A) Bar graph depicts the expression of TNF-α in the different groups. (B) Bar graph displays the protein levels of IL-6 in the four groups. (C) The interference efficiencies of si-GPR41 and si-GPR43 were assessed based on the respective mRNA levels in BV2 cells. (D) Bar graph displays the expression of TNF-α in cells treated with the GPR43 agonist (10 μM). (E) Bar graph displays the protein levels of IL-6 in cells treated with the GPR43 agonist. Data are expressed as the mean±SEM, *P<0.05 vs. the control group, #P<0.05 vs. the LPS and LPS+DMSO groups. DMSO was used as a solvent control. ACE: acetate.