Research Paper Volume 12, Issue 10 pp 8820—8836

LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway

Figure 1. HPRT1 is poorly expressed in the substantia nigra tissues of 6-OHDA-induced PD mice. (A) The expression of HPRT1 in the expression profile of GSE20141 related to PD; (B) The expression of HPRT1 in the expression profile of GSE20168 related to PD. (C) The protein expression of TH in the substantia nigra tissues of 6-OHDA-induced PD mice measured by western blot analysis. (D) Immunohistochemical analysis for the TH positive cells in the substantia nigra tissues of 6-OHDA-induced PD mice (upper × 100, lower × 400); (E) Fluoro-Jade B-stained apoptotic neurons (scale bar = 50 μm). (F) mRNA expression of HPRT1 in the substantia nigra tissues examined by RT-qPCR; (G) Protein expression of HPRT1 in the substantia nigra tissues examined by western blot assay. *p < 0.05. n = 6. Measurement data are by means ± standard deviation. Comparison between two groups was analyzed by independent sample t test.