Research Paper Volume 12, Issue 10 pp 8820—8836

LncRNA H19 diminishes dopaminergic neuron loss by mediating microRNA-301b-3p in Parkinson’s disease via the HPRT1-mediated Wnt/β-catenin signaling pathway

Figure 7. H19 inhibits dopaminergic neuron loss by inhibiting miR-301b-3p. 6-OHDA-induced PD model mice were treated with agomir-miR-301b-3p alone or in the presence of oe-H19. (A) TH positive neurons in the substantia nigra tissues examined by immunohistochemistry (upper × 100, lower × 400). (BE) The mRNA expression of Nurr-1 (B), Pitx-3 (C), Ngn-2 (D) and NeuroD1 (E) in the substantia nigra tissues examined by RT-qPCR. (F) Fluoro-Jade B-stained apoptotic neurons in the substantia nigra tissues (scale bar = 50 μm). *p < 0.05. Measurement data are by means ± standard deviation. Comparison between two groups was analyzed by independent sample t test. n = 6.