Research Paper Volume 12, Issue 5 pp 4322—4336

Sirt1 antisense long non-coding RNA attenuates pulmonary fibrosis through sirt1-mediated epithelial-mesenchymal transition

Figure 1. lncRNA sirt1 antisense (AS) inhibits TGF-β1-induced fibrogenesis in alveolar epithelial cells. (A) Relative expression of sirt1 AS in alveolar epithelial (RLE-6TN) cells with 10 ng/ml TGF-β1 treatment for various time, as indicated. (B) Relative expression of sirt1 AS in RLE-6TN cells infected with adenovirus-mediated overexpression sirt1 AS or adenovirus negative control. (C) CCK-8 assay was used to detect the cell viability of RLE-6TN cells with or without overexpression of sirt1 AS up TGF-β1 treatment. (DH) Relative expression of epithelial-mesenchymal transition (EMT) related markers α-SMA, E-cadherin, collagen1 and fibronectin1 in RLE-6TN cells with or without overexpression of sirt1 AS up TGF-β1 treatment; and densitometry quantified data of above indicated markers, represented as a fold change to β-actin. (I) Immunofluorescence staining showing the overlap of α-SMA and E-cadherin in RLE-6TN cells with indicated treatment. (J) Transwell assay was performed to investigate the migration ability of RLE-6TN cells with indicated treatment. * P<0.05 vs. control group; # P<0.05 vs. TGF-β1+ad-NC group.