Research Paper Volume 12, Issue 6 pp 4866—4878

MiR-519d targets HER3 and can be used as a potential serum biomarker for non-small cell lung cancer

Figure 4. HER3 is a target gene of miR-519d. (A) Identification of conserved miR-519d binding site in 3’-UTR of HER3, using TargetScan. (B) Dual luciferase reporter assay showed that miR-519d significantly suppressed the relative luciferase activity of pmirGLO-HER3-3’UTR in 293 cells. (C) Real time PCR analysis of miR-519d levels in A549 cells transfected with miR-519d mimic. (D) HER3 expression in A549 cells transfected with miR-519d mimics. (E) Real time PCR of miR-519d levels in A549 cells transfected with miR-519d inhibitor. (F) HER3 expression in A549 cells transfected with miR-519d inhibitor. *p<0.05 vs. control, ***p<0.001 vs. control.