Research Paper Volume 12, Issue 5 pp 4558—4572

Long non-coding RNA RHPN1-AS1 promotes tumorigenesis and metastasis of ovarian cancer by acting as a ceRNA against miR-596 and upregulating LETM1

Figure 5. RHPN1-AS1 acts as a sponge for miR-596 to increase LETM1 expression. (A, B) Relative mRNA and protein levels of LETM1 in ES-2 and HEY cells transfected with miR-596-mimics or miR-596 inhibitor. (C) The predicted binding site of miR-596 in the LETM13'-UTR. (D) Luciferase activity in ES-2 and HEY cells co-transfected with WT/mutant LETM1 plasmids with miR-596 mimic or miR-Ctrl. (E) Enrichment of LETM1 by biotin-miR-596. (F, G) LETM1 protein levels in ES-2 and HEY cells transfected with sh-RHPN1-AS1s or pcDNA-RHPN1-AS1. (H) Correlation between RHPN1-AS1 and LETM1 expression in EOC tissues. (I) Expression levels of LETM1, FAK, p-FAK, and AKT, and p-Akt in cells transfected with shRHPN1-AS1#2 and/or miR-596 inhibitor. (J) Expression levels of LETM1, FAK, p-FAK, AKT, and p-Akt in cells transfected with pcDNA-RHPN1-AS1 and/or miR-596 mimic. *P<0.05, **P<0.01.