Research Paper Volume 12, Issue 5 pp 4558—4572

Long non-coding RNA RHPN1-AS1 promotes tumorigenesis and metastasis of ovarian cancer by acting as a ceRNA against miR-596 and upregulating LETM1

Figure 6. LETM1 is involved in RHPN1-AS1-mediated EOC progression. (A, B) CCK-8 assay in ES-2 and HEY cells transfected with shRHPN1-AS1#2 and/or pcDNA-LETM1. (C) Colony formation analysis in EOC cells transfected with shRHPN1-AS1#2 and/or pcDNA-LETM1. (DG) Transwell assay in EOC cells transfected with shRHPN1-AS1#2 and/or pcDNA-LETM1. (H) Western blotting analysis of LETM1, FAK, p-FAK, Akt, p-Akt in EOC cells transfected with shRHPN1-AS1#2 and/or pcDNA-LETM1. (I) Western blotting analysis of LETM1, p-FAK, Akt, p-Akt in ES-2 and SUNE1 cells transfected with pcDNA-RHPN1-AS1 and/or sh-LETM1. (J) A mechanism model for the expression and function of RHPN1-AS1 in EOC. *P<0.05, **P<0.01.