Research Paper Volume 12, Issue 6 pp 5091—5120

Figure 10. In vitro response of whole blood platelets from mice with orthotopic model of breast cancer to increasing concentrations of thrombin. Results are presented as median (horizontal line) and interquartile range (box) (n = 8). The expressions of P-selectin (CD62P) (A), the active form of GPIIb/IIIa (B) and binding of endogenous vWF (C) and endogenous fibrinogen (Fg) (D) on platelets stimulated with low thrombin (0.025 U/ml) (A, C, E, G) or high thrombin (0.25 U/ml) (B, D, F, H) were measured using flow cytometry in non-fixed ‘washed blood’ withdrawn immediately (t₀) or after 2 (t₂), 3 (t₃), 4 (t₄) or 5 weeks (t₅) from the injection of 4T1 cells. Results are expressed as the percent fraction of platelets positive for a given activation marker. More experimental details are given in the Materials and methods section. The statistical significance of differences, estimated with Kruskal-Wallis test followed by the post hoc Conover-Inman all-pairwise comparisons or one-way ANOVA followed by Tukey’s multiple comparisons test, was: P-selectin _thr0.025U/ml, P_1,α < 0.05, 4T1 t₅ = 4T1 t₄ > 4T1 t₃ > 4T1 t₂ > 4T1 t₀; P-selectin _thr0.25U/ml, P_1,α < 0.001, 4T1 t₅ > 4T1 t₄ = 4T1 t₃ > 4T1 t₂ < 4T1 t₀; active form of GPIIb/IIIa _thr0.025U/ml, P1_,α < 0.05, 4T1 t₅ < 4T1 t₄ = 4T1 t₃ > 4T1 t₂ = 4T1 t₀; vWF _thr0.025U/ml, P_1,α < 0.001, 4T1 t₅ = 4T1 t₄ = 4T1 t₃ > 4T1 t₂ = 4T1 t₀; vWF _thr0.25U/ml, P_1,α < 0.01, 4T1 t₅ = 4T1 t₄ > 4T1 t₃ < 4T1 t₂ = 4T1 t₀; Fg_{thr0.025U/ml,} P_1,α < 0.001, 4T1 t₅ = 4T1 t₄ = 4T1 t₃ > 4T1 t₂ = 4T1 t₀; Fg_thr0.25U/ml, P_1,α < 0.05, 4T1 t₅ < 4T1 t₄ = 4T1 t₃ > 4T1 t₂ = 4T1 t₀.