Research Paper Volume 12, Issue 6 pp 5121—5139

EMMPRIN/CD147 plays a detrimental role in clinical and experimental ischemic stroke

Figure 3. CD147 is expressed on brain infiltrating monocytes. (A) Representative dot plot of sham and stroke (72hr) brain shows microglia and infiltrating myeloid cells. Representative dot plot of 72hr stroke brain, gated on myeloid cells, shows Ly6Chi monocytes, Ly6Clo monocytes, and Ly6G neutrophils. (B) Absolute cell counts at 72hr in stroke brain (and sham microglia). (C) Quantified % CD147 positive cells in brain. Representative histogram showing expression of CD147 on microglia (sham and stroke; gray= microglia-specific FMO). Representative histogram showing expression of CD147 on brain-infiltrating myeloid cells after stroke. (D) Quantified CD147+ cell counts from brain, n=6. (E) Quantified %CD147 positive blood cells. Representative histogram showing expression of CD147 on blood cells after stroke. (F) Quantified expression level of MMP-9 on brain-infiltrating myeloid cells (and sham microglia). Representative histogram showing expression of MMP-9 on brain-infiltrating myeloid cells (and sham microglia).