Research Paper Volume 12, Issue 6 pp 5195—5208

Peroxisomal abnormalities and catalase deficiency in Hutchinson-Gilford Progeria Syndrome

Figure 4. Catalase overexpression alleviates oxidative stress in normal and HGPS fibroblasts. (A, B) Quantitative RT-PCR analysis showed the relative expression of catalase in normal and HGPS fibroblasts infected by pBABE-CAT and control pBABE-puro retroviral vectors (Vehicle), respectively. **, p < 0.01. (C, D) Relative fold change of ROS activity measured by DCFDA flow cytometry analysis in normal and HGPS fibroblasts overexpressing catalase. *, p < 0.05, **, p < 0.01. (E) Western blot analysis showed catalase expression in normal and HGPS fibroblasts infected with pBABE-CAT and control vectors (cell passage number = 18). (F) Western blot analysis showed Nrf2 and p16 expression in normal and HGPS fibroblasts infected with pBABE-CAT and control vectors (cell passage number = 18). (G, H) Quantification of Nrf2 and p16 relative expression from Figure 4F. *, p < 0.05, **, p < 0.01, n.s., not significant. All experiments were performed using mid-passage cells between p15 to p25. All experiments were repeated at least three times and representative data were shown as indicated.