Figure 2. Sevoflurane increased Nupr1, C/EBPβ, and IGFBP5 expression and induced apoptosis in HCN-2 neuronal cells. Identical numbers of HCN-2 neuronal cells were exposed to fresh gas (21% O2, 5% CO2, remainder N2) alone or with the addition of 3.4% sevoflurane for 6 h and then subjected to analysis. (A, B) Western blotting for Cleaved-Caspase-3, Bax, and Bcl-2 in 3.4% SEVO-exposed vs. control HCN-2 cells; representative images (A) and quantification (B). (C, D) Annexin V-FITC/PI staining and flow cytometry analysis; representative images (C) and quantification (D). (E, F) Western blotting for Nupr1, C/EBPβ, and IGFBP5 in 3.4% SEVO-exposed vs. control HCN-2 cells; representative images (E) and quantification (F). **p<0.05. N=6.