Research Paper Volume 12, Issue 6 pp 5209—5220

MicroRNA-325-3p prevents sevoflurane-induced learning and memory impairment by inhibiting Nupr1 and C/EBPβ/IGFBP5 signaling in rats

Figure 6. Upregulation of Mir-325-3p expression attenuated sevoflurane-induced learning and memory impairments in neonatal rats. AgomiR-325-3p or agomiR-NC was intracranially injected bilaterally into the hippocampi of neonatal rats (n=6 per group). One day after injection, the rats were exposed to SEVO. (A, B) A novel object recognition test was performed 8 weeks after SEVO exposure. (A) Exploration times during the recognition session for familiar (X) and novel (Y) objects. (B) The discrimination index indicates time spent exploring the novel object relative to total exploration time for both the novel and familiar objects. (CE) An open field test was performed 8 weeks after SEVO exposure. (C) Time spent in the center of the open field during the 5 min exploration period. (D) Total distance traveled during the 5 min exploration period. (E) Traces showing rats’ movements during the 5 min exploration period. (F, G) TUNEL staining in the rat hippocampus; representative images (F) and quantification (G). (H) RT-qPCR for Nupr1 mRNA. (IJ) Western blotting for C/EBPβ and IGFBP5 in the rat hippocampus; representative images (I) and quantification (J). **p<0.05. N=6.