Research Paper Volume 12, Issue 6 pp 5280—5299

m6A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway

Figure 3. TGF-β1 induces fibrosis via upregulating MALAT1 expression in HK2 cells. (A) qRT-PCR analysis of MALAT1 expression in HK2 cells treated with TGF-β1. (B) qRT-PCR analysis of MALAT1 expression in HK2 cells transfected with siMALAT1 or siNC for approximately 48 h. (C) Western blot analyses of E-cad, α-SMA and GAPDH expression in HK2 cells receiving different treatments. (DF) CCK8, EdU and cell migration analyses of the viability, proliferation and migration of HK2 cells receiving different treatments. After pretransfection with siMALAT1 or siNC for 24 h, HK2 cells were treated with 4 ng/mL TGF-β1 for another 48 h. GAPDH was used as a control. *P < 0.05 and **P < 0.01.