Research Paper Volume 12, Issue 6 pp 5280—5299

m6A-induced lncRNA MALAT1 aggravates renal fibrogenesis in obstructive nephropathy through the miR-145/FAK pathway

Figure 5. miR-145 plays a role in regulating FAK expression in HK2 cells treated with TGF-β1. (A and B) qRT-PCR and western blot analyses of FAK expression in HK2 cells transfected with miR-145 mimics, miR-145 inhibitors and their control RNAs. (C) Luciferase reporter analysis of the binding between miR-145 and predicted binding sites in FAK. (D and E) qRT-PCR and western blot analyses of FAK expression in HK2 cells treated with TGF-β1 (F and G). qRT-PCR and western blot analyses of FAK expression in HK2 cells transfected with siFAK or siNC for approximately 48 h. (H) Western blot analyses of E-cad, α-SMA and GAPDH expression in HK2 cells receiving different treatments. (I and J) CCK8, EdU and cell migration analyses of the viability, proliferation and migration of HK2 cells receiving different treatments. (K) Western blot analyses of E-cad, α-SMA and GAPDH expression in HK2 cells receiving different treatments. (L and M) CCK8, EdU and cell migration analyses of the viability, proliferation and migration of HK2 cells receiving different treatments. GAPDH was used as a control. *P < 0.05 and **P < 0.01.