Research Paper Volume 12, Issue 9 pp 7660—7678

HOTAIR drives autophagy in midbrain dopaminergic neurons in the substantia nigra compacta in a mouse model of Parkinson’s disease by elevating NPTX2 via miR-221-3p binding

Figure 5. HOTAIR elicits autophagy via miR-221-3p dependent NPTX2 elevation in PD cell models. The viability of PD MN9D cells measured by the CCK8 assay, *p < 0.05 vs. PD MN9D cells treated with oe-HOTAIR + sh-NC. (A) The ratios of LC3B-I/LC3B-II and LAMP1/LAMP2 along with the P62 expression patterns in PD MN9D cell models measured by Western blot analysis as well as protein grayscale statistics, *p < 0.05 vs. PD MN9D cells treated with oe-NC + sh-NC, #p < 0.05 vs. PD MN9D cells treated with oe-HOTAIR + sh-NC. (B) The viability of PD MN9D cells measured by CCK8 assay, *p < 0.05 vs. PD MN9D cells treated with oe-NC + mimic NC, #p < 0.05 vs. PD MN9D cells treated with oe-HOTAIR + mimic NC. (C) The ratios of LC3B-I/LC3B-II and LAMP1/LAMP2 along with the P62 expression patterns in PD MN9D cell models measured by Western blot analysis as well as protein grayscale statistics, *p < 0.05 vs. PD MN9D cells treated with oe-NC + mimic NC, #p < 0.05 vs. PD MN9D cells treated with oe-HOTAIR + mimic NC. (D) Detection of autophagy levels in MN9D cells by co-localization analysis. (E) Data (mean ± standard deviation) among multiple groups were analyzed using one-way ANOVA and subjected to Tukey’s post-hoc test. The experiment was repeated three times. PD, Parkinson’s disease; HOTAIR, HOX transcript antisense intergenic RNA; miR-221-3p, microRNA-221-3p; NPTX2, neuronal pentraxin II; CCK-8, cell counting kit-8; NC, negative control.