Research Paper Volume 12, Issue 9 pp 7679—7693

Quantitative acetylome and phosphorylome analysis reveals Girdin affects pancreatic cancer progression through regulating Cortactin

Figure 1. Expression of Girdin in pancreatic cancer tissues and cell lines. (A) The mRNA expression of Girdin in pancreatic cancer tissues and adjacent normal tissues was identified by RT-PCR analysis (n=41). (B, C) The protein expression of Girdin in pancreatic cancer tissues and adjacent normal tissues was examined by IHC analysis. (D) Girdin expression in pancreatic cancer cell lines (AsPC-1, BxPC-3, CFPAC-1 and PANC-1) was identified by western blot analysis and RT-PCR. (E) Girdin expression was knockdown by 5 shRNAs and Girdin expression was identified by western blot analysis. (F) AsPC-1 and PANC-1 cells were transfected with shGirdin plasmid, the transfection efficiency was examined by RT-PCR and western blot analysis. Data was shown as mean ± SD. ***P<0.001. The experiments were repeated 3 times.