Research Paper Volume 12, Issue 9 pp 7717—7728

Resveratrol attenuates doxorubicin-induced meiotic failure through inhibiting oxidative stress and apoptosis in mouse oocytes

Figure 2. Effect of RES treatment on spindle morphology and chromosome alignment in DXR-treated oocytes. Oocytes were immunolabeled with anti-α-tubulin antibody to visualize spindle and counterstained with Hoechst to observe the chromosomes. (A) Representative images of spindle morphologies and chromosome alignment in control, DXR-treated and RES-supplemented oocytes. Green, α-tubulin; blue, DNA. Bar = 20 μm. (B) Representative images of chromosomes alignment in control, DXR-treated and RES-supplemented oocytes. Blue, DNA. Bar = 5 μm. (C) Rate of aberrant spindle was recorded control, DXR-treated and RES-supplemented oocytes. (D) Rate of misaligned chromosomes was recorded in control, DXR-treated and RES-supplemented oocytes. All experiments were repeated at least 3 times with more than 30 oocytes examined in each experimental condition. Results were represented as means ± SEMs. *means P < 0.05, **means P < 0.01.