Research Paper Volume 12, Issue 8 pp 7056—7065

CO2 lattice laser reverses skin aging caused by UVB

Figure 1. Effect of CO2 Lattice Laser on Fibroblasts. (A) Cell proliferation analyzed by MTT assay after treatment with 0, 1.25, 3.75 and 6.25 mJ/cm2 CO2 Lattice Laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (B) ROS analyzed by ELISA in cells irradiated by CO2 lattice. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (C) SOD expression analyzed by ELISA in cells irradiated by CO2 lattice laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (D) MDA expression analyzed by ELISA in cells irradiated by CO2 lattice laser. Data are shown as mean ± SEM.** P< 0.05 vs. 0 mJ/cm2 (E, F) Western blotting and RT-PCR of SMAD3 in cells treated with 0, 1.25, 3.75 and 6.25 mJ/cm2 CO2 Lattice Laser; ** P< 0.05 vs. 0 mJ/cm2.