Research Paper Volume 12, Issue 9 pp 7729—7746

Long noncoding RNA LINC00324 promotes retinoblastoma progression by acting as a competing endogenous RNA for microRNA-769-5p, thereby increasing STAT3 expression

Figure 7. Downregulation of miR-769-5p neutralizes the effects of LINC00324 knockdown on RB cells. (A) RT-qPCR analysis was used to quantify the expression of miR-769-5p in Y79 and WERI-RB-1 cells that were transfected with either antagomir-769-5p or antagomir-NC, *P < 0.05 vs. the antagomir-NC group. (B, C) Si-LINC00324 was cotransfected with either antagomir-769-5p or antagomir-NC into Y79 and WERI-RB-1 cells. After transfection, RT-qPCR and western blotting were performed to measure the expression of miR-769-5p and STAT3 protein, respectively. *P < 0.05 vs. the si-NC group, #P < 0.05 vs. group si-LINC00324+antagomir-NC. (DI) A CCK-8 assay, colony formation assay, flow-cytometric analysis, and Transwell migration and invasion assays were conducted to analyze the proliferation, colony formation, apoptosis, cell cycle, migration, and invasiveness of the aforementioned cells. *P < 0.05 vs. the si-NC group, #P < 0.05 vs. group si-LINC00324+antagomir-NC.