Research Paper Volume 12, Issue 10 pp 8913—8922

HIX003209 promotes vascular smooth muscle cell migration and proliferation through modulating miR-6089

Figure 7. HIX003209 induced inflammatory mediators secretion, cell growth and migration via regulating miR-6089 expression. (A) The expression of IL-6 was detected by ELISA assay. (B) The expression of TNF-α was detected by ELISA assay. (C) The expression of IL-1β was detected by ELISA assay. (D) The expression of cyclin D1 was measured by qRT-PCR analysis. (E) Cell proliferation was measured by CCK-8 analysis. (F) miR-6089 overexpression inhibited HIX003209-overexpressing VSMCs migration compared to scramble group. (G) The relative wound breadth remain of two groups was showed.**p<0.01 and ***p<0.001.