Research Paper Volume 12, Issue 9 pp 7761—7773

MicroRNA-1298-3p inhibits proliferation and invasion of glioma cells by downregulating Nidogen-1

Figure 3. Overexpression of miR-1298-3p inhibits proliferation of glioma cells. (A) RT-qPCR of miR-1298-3p in cortical astrocytes cell line HA1800, and human glioblastoma cell lines U87MG, SHG-44 and U251. (B, C) MiR-1298-3p levels analyzed by qRT-PCR in U87MG and SHG-44 cells transfected with miR-1298-3p agonist. (D, E) Cell viability analyzed by CCK-8 assay in U87MG and SHG-44 cells transfected with miR-1298-3p agonist for 72 h. (F, G) Cell proliferation analyzed by colony formation assay; **P < 0.01 compared with the NC group.