Figure 6F–J. High glucose levels and CS exposure induced EMT through the TGF-β signaling pathway. qRT-PCR analyses of Zo1 and vimentin in HSAEpiC (F) cells. (G–J) AECs were treated with 25 mM glucose and 1% CSE for 24 hours, and TGF-β was added 2 hours before the cells were harvested. Western blots of Zo1 and vimentin from BEAS-2B (G) and HSAEpiC (H) cells. qRT-PCR analyses of Zo1 and vimentin in BEAS-2B (I) and HSAEpiC (J) cells. * p<0.05, ** p<0.01 compared with the control group; # p<0.05, ## p<0.01 compared with the CSE group.