Research Paper Volume 12, Issue 9 pp 7985—8000

PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway

Figure 2. Inhibition of PHLDA2 inhibits CRC cell proliferation. (A, B) RT-qPCR was used to assess the knockout efficiency of three pLVX-sh-PHLDA2 knockdown fragments in HCT116 and SW480 cells. (C, D) Western blot was used to assess the knockout efficiency of the sh-PHLDA2 lentivirus vector in HCT116 and SW480 cells. (EH) Cell Counting Kit-8 (CCK8) and colony formation assays were used to assess cellular proliferation. Data are shown as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001.