Research Paper Volume 12, Issue 9 pp 8067—8083

BMAL1 knockdown triggers different colon carcinoma cell fates by altering the delicate equilibrium between AKT/mTOR and P53/P21 pathways

Figure 6. P21 and MDM2 expression status in CRC BMAL1-KD cell lines. (A) Quantitative RT-PCR revealed a significant increase of P21 mRNA in SW480 BMAL1-KD cells (n=6; *p<0.05) but not in HCT116 BMAL1-KD and SW620 BMAL1-KD cells. (B) Western-blot analysis revealed a significant increase of P21 protein in SW480 BMAL1-KD cells (n=4; *p<0.05) but not in HCT116 BMAL1-KD and SW620 BMAL1-KD cells. (C) Western-blot analysis revealed increased MDM2 protein in SW480 BMAL1-KD cells (n=5; *p<0.05) but not in HCT116 BMAL1-KD and SW620 BMAL1-KD cells. Left, a representative immunoblot of different independent experiments is shown. Right, Bar charts represent P21 or MDM2 expression normalized to HSC70. All data are shown as mean ± SEM.