Research Paper Volume 12, Issue 10 pp 8953—8967

Valproic acid-labeled chitosan nanoparticles promote recovery of neuronal injury after spinal cord injury

Figure 6. VA-CN enhanced the integrity of blood spinal cord barrier after SCI. The blood spinal cord barrier integrity was measured by Claudin-5, Albumin, IgG expression at 4 weeks (n=6 per group) and Evans blue extravasation at 24 h (n=4 per group) after injury. (A, E) Claudin-5 immunoreactivity and quantification to the spinal cord of rats (Scale bar: 100 μm), * p<0.05, ** p<0.01. (B, F) Albumin immunoreactivity and quantification to the spinal cord of rats (Scale bar: 100 μm), * p<0.05, ** p<0.01. (C, G) IgG immunoreactivity and quantification to the spinal cord of rats (Scale bar: 100 μm), * p<0.05. (D, H) Evans blue extravasation and quantification in the spinal cord of rats (Scale bar: 50 μm), * p<0.05, ** p<0.01 VS SCI, ## p<0.01 VS Sham, ### p<0.001 VS Sham. (I) Quantification data of Evans blue content in the spinal cord (μg/g), * p<0.05 VS SCI, # p<0.05 VS Sham, ## p<0.01 VS Sham.