Research Paper Volume 12, Issue 9 pp 8137—8150

Overexpression of METTL3 attenuates high-glucose induced RPE cell pyroptosis by regulating miR-25-3p/PTEN/Akt signaling cascade through DGCR8

Figure 5. High-glucose regulated PTEN/Akt signal pathway in RPE cells by downregulating METTL3 and miR-25-3p. (A, B) Western Blot was used to determine the expressions of METTL3, PTEN, p-Akt and Akt in RPE cells. (C) The targeting sites of miR-25-3p and 3’UTR regions of PTEN mRNA were predicted by using the online starBase software. (D, E) Dual-luciferase reporter gene system was employed to validate the binding sites of miR-25-3p and 3’UTR regions of PTEN mRNA. (FI) Western Blot was conducted to determine the expression status of PTEN, p-Akt and Akt in RPE cells. (“Con” means “Control”, “Hg” means “High-glucose”, “OE-M” means “Overexpressed METTL3” and “Mic” means “miR-25-3p mimic”). Each experiment had at least 3 repetitions, the data were collected and represented as Mean ± SD. “*” means p < 0.05 and “**” means p < 0.01.