Figure 6. Nrf2 was required for lycopene-induced prevention against cutaneous papilloma. (A) Nrf2-/- animals were treated as previous study. Physical appearance of representatives of the indicated groups. (B) The incidence of papillomas in different treatment groups (n=6). (C) Average numbers of papillomas per mouse from the indicated groups (n=6). (D) Average numbers of papillomas per mouse in different tumor diameter groups (n=6). (E) Representative H&E staining images of mouse skin in different groups (40×). (Bottom) Quantitative analysis of epidermal thickness in H&E images (n=4). (F, G) The level of 8-OHdG, 4-HNE in mouse skin tissues of the indicated groups (n=4). (H) The GSH/GSSG ratio in mouse skin tissues of the indicated groups (n=4). The data are presented as the mean ± SD. ***p < 0.001 (versus DMBA/TPA).