Research Paper Volume 12, Issue 9 pp 8191—8201

The shared KEGG pathways between icariin-targeted genes and osteoporosis

Figure 7. Icariin inhibits apoptosis of hMSCs by suppressing c-Jun/JNK signaling. (A) Expression of c-Jun, p-c-Jun, JNK, and p-JNK analyzed in icariin-treated hMSCs by western blotting; (B, C) Phosphorylation levels of c-Jun and JNK in hMSCs transfected with c-Jun or JNK plasmids and treated with icariin (10 μg/ml), analyzed by western blotting; (D, E) CCK-8 proliferation assay of icariin-treated hMSCs; (F) Apoptosis analyzed by flow cytometry of icariin-treated hMSCs; (G) Apoptosis analyzed in hMSCs transfected with c-Jun and JNK plasmids, and treated with icariin; (H, I) Bcl-2 and Bax mRNA levels analyzed by qRT-PCR.