Research Paper Volume 12, Issue 9 pp 8221—8240

Identification of SYK inhibitor, R406 as a novel senolytic agent

Figure 3. R406 induces cleavage of caspases in senescent HDFs. (AC) Senescent (S) and non-senescent (NS) HDFs were treated with DMSO, R406 (2, 10 μM), and ABT263 (1 μM) for one day and then western blot assays using anti-caspase antibodies were conducted to determine senolytic effect by apoptosis. (DF) Senescent and non-senescent HDFs were treated with DMSO, R406 (2, 10 μM), and ABT263 (1 μM) for three days and then monitored the level of cleaved-caspases by western blot analyses. (G) Senescent HDFs were respectively treated with single or combination of R406 and ABT263 for one day and then western blot assay with anti-caspase-7 antibody was conducted to determine apoptosis. *p<0.05, **p<0.01, ***P<0.001.