Figure 5. P123-PEG2000-DSPE (Dox) and P123-PEG2000-DSPE inhibited the expression of P-glycoprotein (P-gp) in MCF-7R cells. (A) The mRNA levels of multi-drug resistance (MDR1) gene in MCF-7R cells treated with PBS (control), PEG2000-DSPE, P123-PEG2000-DSPE, Dox, PEG2000-DSPE (Dox) and P123-PEG2000-DSPE (Dox) by qRT-PCR. (B) The protein expression of P-gp in MCF-7R cells underwent various treatments using western blotting. (C) The expression of P-gp in MCF-7R cells underwent various treatments by cell immunofluorescence.