Research Paper Volume 12, Issue 9 pp 8339—8351

Transcription factor YY1 inhibits the expression of THY1 to promote interstitial pulmonary fibrosis by activating the HSF1/miR-214 axis

Figure 4. YY1/HSF1/miR-214/THY1 regulates the proliferation and fibrogenic transformation of HEPFs. (A) The expression of YY1, HSF1, miR-214 and THY1 determined by RT-qPCR, and the expression of YY1, HSF1, and THY1 proteins measured by western blot analysis in the HEPFs transfected with si-NC, si-YY1 or si-YY1 and si-THY1 in combination; (B) The cell viability assessed by CCK8 after transfection with si-NC, si-YY1 or si-YY1 and si-THY1 in combination; (C) The mRNA and protein expression of cell proliferation marker Ki67 and fibrosis biomarkers CoI, CoIII, α-SMA and vimentin in the HEPFs transfected with si-NC, si-YY1 or si-YY1 and si-THY1 in combination measured by RT-qPCR and western blot analysis. Statistical data were measurement data, and presented as mean ± standard deviation. Unpaired t-test was used for comparison between the two groups, and one-way ANOVA with Tukey's post hoc test was employed for comparisons among multiple groups. *, p < 0.05 vs. cells transfected with si-NC, #, p < 0.05 vs. cells transfected with si-YY1. The experiment was independently repeated three times.