Research Paper Volume 12, Issue 10 pp 8968—8986

Figure 3. Dia-Exos impaired HUVEC angiogenesis and survival in vitro. (A) The effects of Dia-Exos on HUVEC proliferation were measured with a CCK-8 assay. (B, C) Flow cytometry was used to quantify the cell cycle distribution. (D) The effects of Dia-Exos on the proliferation-related genes Cyclin D1 and Cyclin D3 were assessed using qRT-PCR. (E) The effects of Dia-Exos on the apoptosis-related genes Bcl-2 and Bax were assessed using qRT-PCR. (F, G) A Transwell migration assay was used to assess the effects of Dia-Exos on HUVEC migration; scale bar: 100 μm. (H–J) A tube formation assay was used to assess the effects of Dia-Exos on HUVEC angiogenesis; scale bar: 200 μm. (K, L) The scratch assay results of the three groups; scale bar: 250 μm. Data are the means ± SDs of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.