Research Paper Volume 12, Issue 10 pp 8968—8986

Inhibition of circulating exosomal microRNA-15a-3p accelerates diabetic wound repair

Figure 5. Inhibition of miR-15a-3p partially reversed the impaired functionality of HUVECs treated with Dia-Exos. (A) MiR-15a-3p levels in the three groups were measured using qRT-PCR. (B) CCK-8 assay results of the three groups. (C, D) Flow cytometry was used to quantify the cell cycle distribution in treated cells. (E) The qRT-PCR results of the proliferation-related genes Cyclin D1 and Cyclin D3. (F) The apoptosis-related genes Bcl-2 and Bax were assessed using qRT-PCR. (G, H) A Transwell migration assay was used to assess the effects of miR-15a-3p inhibition on HUVEC migration; scale bar: 100 μm. (IK) A tube formation assay was used to assess the effects of miR-15a-3p inhibition on HUVEC angiogenesis; scale bar: 200 μm. Data are the means ± SDs of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.