Research Paper Volume 12, Issue 9 pp 8352—8371

Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1

Figure 2. HT29 cell-derived exosomes overexpressing miR-183-5p promote proliferation, migration tube formation abilities and angiogenesis of HMEC-1 cells. (A) EdU assay was applied to detect the proliferation of the HMEC-1 cells following treatment with HT29-Exo and miR-183-5p mimic (Scale bar = 50 μm); (B) HMEC-1 cell migration was detected by Transwell assay after treatment of HT29-Exo and miR-183-5p mimic (Scale bar = 50 μm); (C) tube formation abilities of HMEC-1 cell were detected by tube formation assay after treatment of HT29-Exo and miR-183-5p mimic (Scale bar = 100 μm); (DE) expression of angiogenesis-related proteins (VEGFA, VEGFAR2, ANG2, PIGF, MMP-2 and MMP-9) in HMEC-1 cells after treatment of HT29-Exo and miR-183-5p mimic was also detected by western blot analysis; * p < 0.05 compared with the NC-mimic + PBS group, # p < 0.05 compared with the NC-mimic + HT29-Exo, or miR-183-5p mimic + PBS groups. Measurement data were presented as mean ± standard deviation; comparisons among multiple groups were assessed by one-way analysis of variance. Cell experiment was repeated three times.