Research Paper Volume 12, Issue 9 pp 8352—8371

Exosomal miR-183-5p promotes angiogenesis in colorectal cancer by regulation of FOXO1

Figure 3. Overexpression of miR-183-5p promotes proliferation, migration and tube formation abilities of HMEC-1 cells by targeting FOXO1. (A) EdU assay was applied to detect proliferation of HMEC-1 cells after treatment of miR-183-5p mimic and rAd-FOXO1 (Scale bar = 50 μm); (B) HMEC-1 cell migration was detected by Transwell assay after treatment of miR-183-5p mimic and rAd-FOXO1 (Scale bar = 50 μm); (C) tube formation abilities of HMEC-1 cell were detected by tube formation assay after treatment of miR-183-5p mimic and rAd-FOXO1(Scale bar = 100 μm); (DE) expression of angiogenesis-related proteins (VEGFA, VEGFAR2, ANG2, PIGF, MMP-2 and MMP-9) and FOXO1 in HMEC-1 cells after treatment of miR-183-5p mimic and rAd-FOXO1 was also detected by western blot analysis; * p < 0.05 compared with the NC-mimic + GFP group, # p < 0.05 compared with the miR-183-5p mimic + GFP group; Measurement data were presented as mean ± standard deviation; comparisons among multiple groups were assessed by one-way analysis of variance. Cell experiment was repeated three times.