Research Paper Volume 12, Issue 9 pp 8372—8396

KIF23 activated Wnt/β-catenin signaling pathway through direct interaction with Amer1 in gastric cancer

Figure 6. KIF23 promoted GC cells proliferation via binding with Amer1 and disturbing its distribution. Effects of cotransfection of KIF23-siRNA and Amer1-siRNA or KIF23and Amer1 overexpression plasmids on cell growth (A) and colony formation assay (B). (C) Western blot analysis of the activity of Wnt/β-catenin signaling pathway and its target proteins after cotransfection of KIF23-siRNA and Amer1-siRNA or KIF23 and Amer1 overexpression plasmids. (D) Q-PCR analysis of effects of cotransfection of KIF23-siRNA and Amer1-siRNA or KIF23and Amer1 overexpression plasmids on Wnt targets Axin2 and Lgr5. IHC (E) and IF (F) were used to analyze the expression and distribution of KIF23 and Amer1 in GC and adjacent normal tissues.