Research Paper Volume 12, Issue 10 pp 9000—9013

The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress

Figure 2. SIRT1 potentiated the BMP2-induced expression of chondrogenic differentiation makers. (A) The Sox9, Col2a1, and aggrecan protein levels in C3H10T1/2 cells infected with Ad-GFP, Ad-SIRT1, Ad-BMP2, or Ad-BMP2+Ad-SIRT1 for 5 days. (B) The Sox9, Col2a1, and aggrecan protein levels in C3H10T1/2 cells infected with Ad-GFP, Ad-SIRT1, Ad-BMP2, or Ad-BMP2+Ad-SIRT1 for 7 days. (C) The Sox9, Col2a1, and aggrecan protein levels in C3H10T1/2 cells infected with Ad-GFP, Ad-SIRT1, Ad-BMP2, or Ad-BMP2+Ad-SIRT1 for 9 days. All of the relative protein expression level results were compared to those of GAPDH using Quantity one. The data are denoted as the mean ± SD.*:p < 0.05 vs. Ad-GFP; #:p < 0.05, Ad-BMP2 group vs. Ad-BMP2+Ad-SIRT1 group.