Research Paper Volume 12, Issue 10 pp 9000—9013

The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress

Figure 3. The C3H10T1/2 cells were cultured in 6-well plates after being infected with Ad-GFP, Ad-SIRT1, Ad-BMP2, or Ad-BMP2+Ad-SIRT1. (AC) The Sox9 mRNA levels of C3H10T1/2 cells were measured after 5, 7 and 9 days using real-time PCR. (EG) The Col2a1 mRNA levels of C3H10T1/2 cells were measured after 5, 7 and 9 days using real-time PCR. (D, H) Alcian blue staining was used to observe the secretion of extracellular matrix after the 5 and 7 days of Ad-GFP, Ad-SIRT1, Ad-BMP2, and Ad-BMP2+Ad-SIRT1 infection. The dyed 12-well plates were photographed by a camera attached to a light microscope (100X). All of the relative mRNA expression level results were compared to those of GAPDH using Quantity one. The data are denoted as the mean ± SD.*:p < 0.05 vs. Ad-GFP; #:p < 0.05, Ad-BMP2 vs. Ad-BMP2+Ad-SIRT1.