Research Paper Volume 12, Issue 10 pp 9000—9013

The role of SIRT1 in BMP2-induced chondrogenic differentiation and cartilage maintenance under oxidative stress

Figure 6. SIRT1 inhibited the decomposition of extracellular matrix in BMP2-induced-C3H10T1/2 cells under oxidative stress. (A, B) Immunofluorescence analysis of the expression of Col2a1 and aggrecan among the Ad-BMP2, Ad-BMP2+H2O2, and Ad-BMP2+Ad-SIRT1+H2O2 groups (200X). The Col2a1 and aggrecan proteins were labeled in red, and the nuclei were labeled in blue by DAPI staining. (C, D) The expression of Sox9, Col2a1, and aggrecan proteins decreased in the Ad-BMP2+H2O2 group compared with the Ad-BMP2 group, while the expression of Sox9, Col2a1, and aggrecan proteins increased in the Ad-SIRT1+Ad-BMP2+H2O2 group compared with that of the Ad-BMP2+H2O2 group. The expression of MMP2 increased in the Ad-BMP2+H2O2 group compared with that in the Ad-BMP2 group, while the expression of MMP2 in the SIRT1+BMP2+H2O2 group was significantly lower than that of the Ad-BMP2 group. (E, F) The expression of Ac-p65 and p65 among Ad-BMP2, Ad-BMP2+H2O2, and Ad-BMP2+Ad-SIRT1+H2O2 groups. The data are denoted as the mean ± SD. *: p < 0.05, Ad-BMP2+H2O2 vs. Ad-BMP2; #: p < 0.05, Ad-BMP2+Ad-SIRT1+H2O2 vs. Ad-BMP2+H2O2.