Research Paper Volume 12, Issue 10 pp 9066—9084

Overexpression of miR-142-5p inhibits the progression of nonalcoholic steatohepatitis by targeting TSLP and inhibiting JAK-STAT signaling pathway

Figure 3. The expression of miR-142-5p and TSLP and the function of miR-142-5p to TSLP in the liver of mouse model. (A) The relative expression of miR-142-5p in the liver of different group. (B) Relative mRNA expression of TSLP in the liver of different mouse group. (C) Protein expression of TSLP in the liver of different mouse group; WT group: wild-type mice fed a chow diet (CD); blank group: Ldlr−/− mice fed an high-fat diet (HFD) treated with phosphate buffer saline (PBS); NC group: Ldlr−/− mice fed an HFD treated with control agonis; A142 group: Ldlr−/− mice fed an HFD treated with agonist of miR-142-5p; A142-C group: Ldlr−/− mice fed an HFD treated with miR-142-5p agonist and JAK-STAT signaling pathway activator-colivelin; A142-TSLP group: Ldlr−/− mice fed an HFD treated with miR-142-5p agonist and AAV/TSLP. AAV: adeno-associated virus. n=6. *P < 0.05; **P < 0.01, compared with WT group; #P< 0.05; ##P < 0.01, compared with NC group; ^P < 0.05, compared with A142 group. All data were means ± SD.