Figure 2. TNF down-regulates PAK4 and cdc42 expression in HUVECs. (A) Western blotting of HUVECs incubated with 100 ng/ml TNF for up to 24 hours, analyzed using anti-PAK4, anti-cdc42, anti-β-actin, and control anti-tubulin antibodies. (B) Confocal microscopy of HUVECs incubated 0 and 24 h with 100 ng/ml TNF, and stained for PAK4 and F-actin; scale bar = 10 μm. Cells were counter stained with DAPI.