Research Paper Volume 12, Issue 10 pp 9085—9102

lncRNA ZFAS1 promotes lung fibroblast-to-myofibroblast transition and ferroptosis via functioning as a ceRNA through miR-150-5p/SLC38A1 axis

Figure 6. lncRNA ZFAS1 regulates FMT activation via the miR-150-5p/SLC38A1 axis in HFL1 cells. (A, B) Western blot was performed to detect the expression of SLC38A1 in HFL1 cells; (C) BrdU staining was applied to analyze cell viability; (D) Wound healing assay was used to detect the migration of HFL1 cells; (E, H) The expression levels of α-SMA and E-cadherin in HFL1 cells were measured by immunofluorescence staining; (F, G) Western blot was performed to detect the expression levels of E-cadherin, collagen I, FN1 and α-SMA. ΔΔP<0.01, compared with NC group; **P<0.01, compared with the control group; ##P<0.01, compared with the TGF-β1 group; ▲▲P<0.01, compared with the TGF-β1+si-SLC38A1 group.