Figure 7. Improvement of inflammation and promotion of proteoglycan anabolism. (A) immunohistochemical analysis of senescence and inflammation-related markers and safranine O staining after 21-day pellet culture. Scale bar: 200 μm. (B) mRNA expression analysis by qPCR for CDKN1A, CDKN2A, MMP13, ADAMTS5, IL1β, IL6, COLII, ACAN and SOX9. (C) protein level analysis using Immunoblotting for C-Caspase3, p21, MMP13, ADAMTS5, IL1β, COLII, ACAN and SOX9. Data are shown as mean ± standard deviation. N = 3 per group.