Research Paper Volume 12, Issue 13 pp 12750—12770

Navitoclax (ABT263) reduces inflammation and promotes chondrogenic phenotype by clearing senescent osteoarthritic chondrocytes in osteoarthritis

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Figure 8.

Senescent cells clearance by treatment with ABT263 after rat DMM surgery attenuates the injury of post-traumatic OA. (A) Schematic of the time course for the experiments in b–d. Male SD rat that underwent DMM surgery were injected intra-articularly every other day with vehicle (Veh) or ABT263 for two weeks and evaluated as indicated. (B) Representative images of Hematoxylin and eosin staining and safranin O and methyl green staining of the medial femoral condyle and medial tibial plateau in rat right knee. Scale bar: top: 500 μm; bottom: 100μm. (C) OARSI scores of the knee joints of rat with DMM or sham surgery. N = 4 per group. (D) Sagittal micro-CT and three-dimensional reconstruction images of the hind knee joint from different groups. ABT263 of 1.0 mM was corresponded to the results presented in B, C and D; Data are shown as mean ± standard deviation. Scale bar: 2 mm.